Immunohistochemical antigen demonstration in plastic-embedded lymphoid tissue.

Reagents for the Demonstration of Bovine Vih.al Diarrhea Virus (bvdv) Antigen in Lymphoid Tissue

Tissue demonstration of carcinoembryonic antigen (CEA) in ulcerative colitis.

An immunoperoxidase technique was used to demonstrate carcinoembryonic antigen (CEA) in sections of proctocolectomy specimens from nine cases of ulcerative colitis in some of which carcinoma and premalignant change had developed. Carcinoma and premalignant change stained positively for CEA in contrast with benign mucosa, whether or not inflammatory dysplasia was present.

Immunohistochemical Localization of Membrane and a - Granule Proteins in Plastic - Embedded Mouse Bone Marrow

Using an immunoperoxidase technique that permits optimal antigen localization at the light microscope level. we have detected two platelet a-granule constituents and three platelet membrane glycoproteins in mouse bone marrow megakaryocytes and in murine megakaryocyte colonies grown in soft agar culture for three to seven days. Using polyclonal antibodies prepared against human platelet proteins...

Plastic-embedded protein crystals.

Rapid vitrification followed by the replacement of the vitrified water by a solvent (freeze substitution) and then resin is a widely used procedure for preparing biological samples for electron microscopy. The resulting plastic-embedded samples permit convenient room-temperature sectioning (microtomy) and can yield well preserved cellular structures. Here this procedure has been applied to crys...

Immunohistochemical demonstration of Toxoplasma gondii.

A peroxidase-antiperoxidase method that demonstrates both free and encysted toxoplasmata in routinely prepared histologic sections is described. This technic and ultrastructural study were employed to confirm the presence of Toxoplasma gondii in the infected tissues of an immunosuppressed patient who had postmortem examination. An immunohistochemical method may be an aid in the diagnosis of tox...